Preparation of Oxygen Meter Based Biosensor for Determination of Triglyceride in Serum

A method is described for preparation of a dissolved oxygen meter (make Aqualytic, Germany) based triglyceride biosensor employing a polyvinyl chloride (PVC) membrane bound lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase The biosensor measures dissolved O2 utilized in the oxidation of triglyceride (TG) by membrane bound lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO), which is directly proportional to (TG) concentration. The biosensor showed optimum response within 10-15 sec at pH 7.5 and 39.5 oC. A linear relationship was obtained between the (TG) concentration from 5mM to 20mM and oxygen consumed (mg/L). The biosensor was employed for determination of triglyceride in serum. The within and between batch coefficient of variation (CV) were < 2.18 % and < 1.7% respectively. The minimum detection limit of the biosensor was 0.35 mM. A study of interference revealed that ascorbic acid, cholesterol and bilirubin caused 13%, 15%, and 12% interference, respectively .The biosensor is portable and can be used outside the laboratory.